Inertness & Durability Combined in a new HPLC Column
Generally, silica based columns are mechanically stable and provide high efficiencies, however, they cannot be used under alkaline conditions and their residual silanol groups tend to adsorb organic bases.
InertSustain employs a radically new type of silica, in which the surface of the silica is uniquely modified, enabling precise control of the silica properties. InertSustain inherits the advantages of all the current Inertsil HPLC columns (e.g., extremely low operating back pressure, superior inertness to typically any analytes, high efficiency and compatibility with a wide range of solvents), but now can be used for wide pH analysis with consistent performance from column to column and lot to lot.
Radically New Silica Gel
It is not possible to end-cap 100 % of residual silanols using traditional chemical modification procedures. GL Sciences studied the possibility of developing a radically new type of silica, a silica that would provide both high inertness (base deactivation) and durability at a wide range of pH.
1st Stage [Synthesis of “Evolved Surface Silica
As the adsorption sites and silanol distribution are strictly controlled from the synthesis procedure of the Evolved Surface Silica, InertSustain delivers unmatched inertness to virtually any type of analyte with high durability to acidic, basic mobile phase conditions.
2nd Stage [Chemical Bonding of ODS group]
The introduction of Evolved Surface Silica and our cutting-edge chemical bonding technology make InertSustain compatible with 100 % aqueous mobile phases, while maintaining strong non-polar retentivity.
3rd Stage [Complete End-capping]
GL Sciences’ complete end-capping technology allows InertSustain to provide high efficiency and superior peak shape even to those well-known strong adsorptive analytes.
Developing a new method is a time-consuming operation. On top of this selecting an HPLC column plays a major role to achieve the desired separation for your critical analysis.
The InertSustain series are offered with C18, C8, Phenyl and Aminopropyl phases, which cover an extended range of selectivity with extremely inert packing materials to answer your diffi cult method development.
InertSustain™ C18 HPLC Column
Tailing of peaks or adsorption of peaks can be experienced when a column has residual adsorption sites. Such phenomenon would not be observed on InertSustain as the silanols are completely end-capped, having a neutral silica surface.
Long Column Lifetime
Extreme Low Operating Back Pressure
pH Range from 1 ～ 10
Experience The InertSustain™ (Inertness & Sustainability)
Highly end-capped ODS column such as InertSustain C18 offers an opportunity to fl ush out contaminants from the column surface easily using an organic solvent. Coffee melanoidins are brown heterogeneous polymers contained in coffee. Its components are not clarifi ed yet, but it is considered to contain several ionic compounds, which a poorly end-capped column will adsorb those ionic compounds leading to short column lifetime.
As for ODS column, which is commonly used for HPLC and LC/MS/MS, its inertness has an infl uence not only on peak shape but also detection sensitivity and durability. It is highly recommended to use highly end-capped column which provides good peak shape for both basic and acidic compounds such as InertSustain C18.
Quality and Performance of InertSustain™
To maintain precise product reproducibility, strict chromatographic tests for inertness, durability, theoretical plates and reproducibility of retention time are employed.
X Select CSH C18
ZORBAX Eclipse Plus C18
X Select CSH C18
ZORBAX Eclipse Plus C18
X Select CSH
Wide pH compatability with Long Column Lifetime
As shown in the experiment below, due to the introduction of Evolved Surface Silica, InertSustain C18 maintained high effi ciency and peak shape
for 300 hours while other “wide pH” column brands failed.
Extremely Low Operating Back Pressure
Due to the introduction of Evolved Surface Silica, InertSustain C18 offers a signifi cantly lower operating back pressure without sacrificing efficiency. As shown below, InertSustain C18 produces the lowest back pressure than other brands columns in the industry.
Reliable Reproducibility, Performance and Quality
Rigorous quality control of physical properties and strict chromatographic tests for inertness and selectivity, contribute to the production of
InertSustain C18 with an outstanding reproducibility and long column lifetime.
Superior peak shape with highly
reproducible chromatograms can
be obtained even for those strong basic compounds.
Reliable reproducibility and
same retention behavior between various particle size columns
About GL Sciences
GL Sciences Inc. was established in 1968 in Japan and is now one of the leading suppliers of HPLC and GC consumables in Japan. GL Sciences began exporting Inertsil HPLC columns into the United States on a large scale in the early 1990s, and their primary trademarked product, Inertsil, quickly became recognized as on of the very best columns on the market.
An outline of InertSustain
Get Your Free C18 HPLC Column Sample Today!
f you would like to evaluate our InertSustain column please provide the column phase and dimensions you are interested in along with your shipping address and we will sign you up for this promotion.
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