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Get Your Free InertSustain HPLC C18 Column

HPLC, LC/MS Column Catalog

Put GL Sciences columns to the test on your own compounds risk-free.  Download catalog & claim your free column.


Inertness and durability combined in a new HPLC column

GL Sciences newly developed technologies has enabled the modification of pore size, and its homogeneity, along with the amount of silanol groups of the base silica to be adequately controlled for each ligand in a variety of phases and applications.

This evolved surface silica (ES Silica) allows GL Sciences InertSustain™ Series to have a wide portfolio with extremely inert, chemical sustainability and batch to batch reproducibility for state-of-the-art analysis.


While other columns show poor peak shape, insufficient separation efficiency often with secondary interactions, and unsuitable physical properties of base silica that result in poor reproducibility. 

InertSustain™ Series delivers symmetric peaks with high efficiency.

Can be used for wide pH analysis with consistent performance

Superior inertness to typically any analytes

High efficiency

Extremely low operating back pressure

Compatibility with a wide range of solvents


“The columns are stable in a wide range of pH and perfectly suitable for separations followed by MS detection.  Compared with other columns such as Synergy Hydro-RP (from Phenomenex) or several columns from Waters dedicated for the separation of hydroxy acids, the separation and the peak shape obtained on the GL Sciences AQ-C18 column were better.”   

Dr. Serban Moldoveanu / Principal Scientist

R.J. Reynolds

Trusted By 

Leading Brands


InertSustain™ C18 Column

Tailing of peaks or adsorption of peaks can be experienced when a C18 column has residual adsorption sites. Such phenomenon would not be observed on InertSustain as the silanols are completely end-capped, having a neutral silica surface.

High Quality & Performance



pH Analysis


Low Back



Reliable Reproducibility


High Quality & Performance

Experience The InertSustain™ (Inertness & Sustainability)

Highly end-capped ODS column such as InertSustain C18 column offers an opportunity to flush out contaminants from the column surface easily using an organic solvent. Coffee melanoidins are brown heterogeneous polymers contained in coffee. Its components are not clarified yet, but it is considered to contain several ionic compounds, which a poorly end-capped column will adsorb those ionic compounds leading to short column lifetime.


As for ODS column, which is commonly used for HPLC and LC/MS/MS, its inertness has an influence not only on peak shape but also detection sensitivity and durability. It is highly recommended to use highly end-capped column which provides good peak shape for both basic and acidic compounds such as InertSustain C18 column.

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Quality and Performance of InertSustain™ C18 Column

To maintain precise product reproducibility, strict chromatographic tests for inertness, durability, theoretical plates and reproducibility of retention time are employed.

Basic Compounds

InertSustain C18 Column

Basic Compound InertSustain C18.jpg

X Select CSH C18

Basic Compound X-Select.jpg

ZORBAX Eclipse Plus C18

Zorbax  Basic Compound.jpg
Conditions - Basic Compound.jpg

Acidic Compounds

InertSustain C18


X Select CSH C18


ZORBAX Eclipse Plus C18


Chelating Compound

InertSustain C18


X Select CSH


Wide pH Compatibility with Long Column Lifetime

As shown in the experiment below, due to the introduction of Evolved Surface Silica, InertSustain C18 maintained high effi ciency and peak shape for 300 hours while other “wide pH” column brands failed.


Extremely Low Operating Back Pressure

Due to the introduction of Evolved Surface Silica, InertSustain C18 offers a signifi cantly lower operating back pressure without sacrificing efficiency. As shown below, InertSustain C18 produces the lowest back pressure than other brands columns in the industry.


Reliable Reproducibility, Performance and Quality

Rigorous quality control of physical properties and strict chromatographic tests for inertness and selectivity, contribute to the production of InertSustain C18 with an outstanding reproducibility and long column lifetime.


Superior peak shape with highly

reproducible chromatograms can

be obtained even for those strong basic compounds.


Reliable reproducibility and

same retention behavior between various particle size columns